Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 Anti-GATA4 [6H10] (Mouse monoclonal) Thermo Fisher Scientific

Invitrogen Anti-Caspase 9 (Cleaved Asp315) Polyclonal, Catalog # PA5-17913. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunoprecipitation (IP) applications.

To elaborate, I am working with A375 (human melanoma), SQ20B (human squamous h&n carcinoma), HT1080 (human 2018-08-31 Anti-Caspase 9 Antibody, active (cleaved) form Chemicon®, from rabbit; find Sigma-Aldrich-AB3629 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems. We report an approach to manufacturing interleukin-10 (IL-10)–loaded EVs (IL-10+ EVs) by engineering macrophages for treating ischemic acute kidney injury (AKI). Delivery of IL-10 via EVs enhanced not only the stability of IL-10, but also its targeting to the kidney (1: 2000; Catalogue No. ab13847, Abcam Bio-tech., Cambridge, MA, USA), rabbit anti-mouse B-cell lymphoma-2 (Bcl-2) polyclonal antibody (1: 2000, Catalogue No. ab59348, Abcam Bio-tech., Cambridge, MA, USA), rabbit anti-mouse Bcl-2 associated protein X … Protein expression for active caspase 3 (ab13847; Abcam, Cambridge, MA) in whole lungs was measured with immunoblotting with available antibodies, as previously described . The intensity of the bands was normalized to the intensity of a reporter protein (actin) with … For immunohistochemistry apoptosis and DNA damage, quantitation was done using antibodies against cleaved caspase 3 (Abcam, ab13847) or γH2AX (clone JBW301; Millipore), respectively. For Western blotting, antibodies against PAXX (Abcam, ab126353) and β-actin (Sigma, A5316) were used.

Current treatment for glioblastoma multiforme (GBM) fails to address its highly infiltrative nature; treatment often leaves behind microscopic neoplastic satellites, resulting in eventual tumor recurrence. Here we report polymeric nanoparticle-engineered human adipose-derived stem cells (hADSCs) overexpressing the cancer-specific TNF-related apoptosis-inducing ligand for targeting and Maternal sevoflurane exposure during pregnancy is associated with increased risk for behavioral deficits in offspring. Several studies indicated that neurogenesis abnormality may be responsible for the sevoflurane-induced neurotoxicity, but the concrete impact of sevoflurane on fetal brain development remains poorly understood. dilution (caspase3, ab13847; caspase9, ab32539; p65, ab16502; IKKα, ab178870; IκBα, ab109300; Abcam, Cambridge, MA, USA) overnight. After washing the cells with PBS, we incubated the cells with fluorescent secondary antibody dilution (Sigma-Aldrich, St. Louis, MO, USA) for 1 hour in the dark. We then washed the cells with PBS 2020-01-06 · Background Ovarian cancer (OC) is a common fatal malignant tumor of female reproductive system worldwide.

Protein Names.

ab13847 recognizes a cleaved form of Caspase 3 (~17 kDa) after apoptosis has been induced in wildtype cells and not Caspase 3 knockout cells. Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application.

ab13847; 1:2,000; Abcam), anti-PAI-1 (cat. no. ab66705; 1:2,000; Abcam), anti-TNF-α (cat.

We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. If you have written or know of another publication, please do let us know!

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunoprecipitation (IP) applications.

#ab32124, Abcam, 1:1000 dilution), as mentioned in Characterization of engineered exosomes. caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat. no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C. Sec-ondary antibodies including goat anti-rabbit (ab205718, … The purpose of this study was to verify the effect of berberine (BBR) on endoplasmic reticulum stress (ERS) and apoptosis of intestinal epithelial cells (IECs) in mice with ulcerative colitis (UC). BALB/c mice were randomly divided into five groups as follows: blank control, model, and low-, medium-, and high-dose BBR. A dextran sodium sulfate- (DSS-) induced model of UC was prepared, and the 2018-07-13 These PVDF membranes were incubated with the antibodies against Caspase‐3 (Abcam, 1:1000, ab13847), ATP7A (Abcam, 1:1000, ab13995), ATP7B (Abcam, 1:1000, ab124973), and β‐actin (Abcam, 1:1000, ab8227) overnight at 4°C and further incubated with HRP‐conjugated secondary antibody (1:6000).
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Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data.

After washing with PBS with 1% Tween-20, the membrane was incubated with respective and species-speciﬁc Horse Radish Peroxidase-labeled secondary antibody Anti-active Caspase 3 antibody (ab13847) Host Organism Rabbit Immunogen Organism Human Isotype.
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https://www.bioz.com/result/expose mouse and rabbit specific hrp dab detection ihc kit/product/Abcam. Average 97 stars, based on 3 article reviews. Price from $9.99 to $1999.99.

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caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat. no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C. Sec-ondary antibodies including goat anti-rabbit (ab205718, …

This can potentially be accomplished by either blocking blood monocyte recruitment to the plaque or increasing macrophage apoptosis and emigration. Digital data. Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data. I 've been having some issues with detecting cleaved caspase 3, 7 and 9 in my w.blots. To elaborate, I am working with A375 (human melanoma), SQ20B (human squamous h&n carcinoma), HT1080 (human 2018-08-31 Anti-Caspase 9 Antibody, active (cleaved) form Chemicon®, from rabbit; find Sigma-Aldrich-AB3629 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. Recently, extracellular vesicles (EVs) have been attracting strong research interest for use as natural drug delivery systems.

Mar 7, 2017 Cathepsin B (ab33538) and cleaved caspase 3 (for immunofluorescence) antibody (ab13847) was purchased from Abcam. Mouse monoclonal

Sapphire Bioscience, ab13847 Rabbit; polyclonal 1:100 (4 wk); 1:200 (12 mo) γ-Immunoglobins heavy and light chains Antirabbit IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate Invitrogen, A-11034 Goat; polyclonal 5 μg/mL γ-Immunoglobins heavy and light chains Antibody Information. Proper Citation: (Abcam Cat# ab13847, RRID:AB_443014) Target Antigen: active Caspase 3, anti- active Caspase 3. Clonality: polyclonal antibody. Comments: validation status unknown, seller recommendations provided in 2012: Flow Cytometry; Immunocytochemistry; Immunohistochemistry; Western Blot; Flow Cytometry, The following primary antibodies were used: Anti-vimentin (cat. no. ab193555; 1:1,000; Abcam), anti-E-cadherin (cat.

Santa Cruz. Sc22948. IF. Anti-CD3 (. Alexa Fluor®. 647-conjugated).